ISHS Acta Horticulturae 488: XI International Symposium on Apricot Culture
IDENTIFICATION OF PLUM X APRICOT INTERSPECIFIC HYBRIDS USING ISOENZYME POLYMPRPHISM |
| Authors: | A.G. Manganaris, A. Mainou, A. Goudaras, C. Ledbetter |
| Keywords: | Isoenzymes, apricots, plums, interspecific hybrids, identification |
| Abstract:
The purpose of this work was to find isoenzyme markers for plum x apricot (plumcot) hybrids to uniquely identify hybrid seedlings from plum x apricot crosses. Ten Japanese plum cultivars (Prunus salicina), 17 apricot cultivars (P. armeniaca), seven plum x apricot (plumcot) hybrids, which were derived from the breeding program of Agricultural Research Service in Fresno and two complex hybrids (Pluot and Aprium) were examined electrophoretically. Starch gels of three buffer systems and acrylamide gels were used for electrophoretic separation of twenty enzyme systems. The most informative enzymes for identifying plumcots were peroxidase (PRX-2, PRX-Cathodal), acid phosphatase (ACP-2) and shikimate dehydrogenase (SKD-1, SKD-2). In addition superoxide dismutase bands SOD-1c and SOD-1d and phosphoglucomutase PGM-2b showed activity only in plums and in plumcots. The existing polymorphism allows to distinguish individually all seven examined plumcots. | |
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