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ISHS Acta Horticulturae 486: II International Symposium on Ornamental Palms & other Monocots from the Tropics

ANALYSIS OF GENETIC BIODIVERSITY IN PALMAE BY DNA MARKER TECHNOLOGY

Authors:   W. Rohde, J. Dowe, A. Kullaya, A. Santos, J. Rodriguez, E. Ritter
Keywords:   breeding, Cocos nucifera L., germplasm, ISTR analysis, population genetics
Abstract:
Various PCR-based DNA marker technologies have been applied to selected palm species for the characterization of genetic diversity within the Palmae for general taxonomic purposes. In addition, genotypes of the coconut palm Cocos nucifera L. as one important oil-producing perennial crop plant of the tropics were analyzed for biodiversity using RAPD, microsatellite primer-primed PCR and the newly developed ISTR technology. The results from analyses of the genetic relationships among genotypes from different palm species were in line with the expectations since genotypes of the same species, the same genus, and the same subfamily clustered together and formed related subclusters in most cases. A more extended analysis which was performed on coconut germplasm collected world-wide from coconut-growing areas, revealed the presence of two distinct groups (Afroindian and Pacific germplasm) and provided evidence for the human-assisted dissemination of coconut. Genetic analysis of single coconut types confirmed that populations of the Tall variants show a higher degree of heterogeneity as compared to the Dwarf types. The currently available data stress the importance of DNA marker application in the management of germplasm collections and in breeding programmes.

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