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ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics

AGROBACTERIAL TRANSFORMATION AND TRANSFER OF THE ANTIFREEZE PROTEIN GENE OF WINTER FLOUNDER TO THE STRAWBERRY

Authors:   A.P. Firsov, S.V. Dolgov
Abstract:
A protocol for agrobacterial transformation of garden strawberry leaf explants was developed. The strain A. tumefaciens A 281 (vector pPCV730) is the most virulent for strawberries giving a transformation frequency of 2.7%. Strain GV3101 with the plasmid-helper pMP90RK (vector series pPCV) is less efficient, the transformation frequency being 1.0–1.7%. On the basis of this protocol, transgenic plants were obtained with the antifreeze protein of winter flounder (AFP). The integration of sequences of genes NPT II and AFP was confirmed by PCR analysis.
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