ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
MOLECULAR MARKERS IN EARLY SEEDLING TESTS FOR SCAB AND MILDEW IN APPLES |
| Authors: | A. Urbanietz, H. Schmidt, F. Dunemann |
| Keywords: | genes, DNA extraction, RAPD, SCAR |
| Abstract:
In a breeding programme for the combination of scab, mildew and Nectria resistance PCR-based markers were used to preselect young seedlings of six progenies for resistance to scab and mildew. Results were compared with scab data after artificial inoculation and natural mildew attack in the greenhouse at the end of the first and second year. Data for the RAPD scab marker OPA 15-900 were in good agreement with results from inoculation, except for progeny 95/8. This family was derived from COOP 8, which has neither the ƒf marker OPD20-600 nor OPA15-900, and obviously is a resistant recombinant genotype. The mildew resistance gene Pl1 was screened with the two flanking markers SCAR AT20-450 and RAPD OPD2-1000. At the end of the first year there was good agreement with infection data for three progenies, two families did not show resistance according to greenhouse infection and markers. Another progeny was totally infected in the greenhouse; however, the marker was present in more than 50% of the plants. This will be rechecked in the greenhouse at the end of the second season. Selection for a marker, instead of relying on natural or artificial infection, could be especially valuable for mildew, which cannot be screened satisfactorily in seed trays. | |
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