ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
PCR ANALYSIS OF SELF-INCOMPATIBILITY ALLELES IN APPLE APPLIED TO LEAVES, SEED EMBRYOS AND IN VITRO SHOOTS |
| Authors: | G.A. Janssens, A.M. Van Haute, J. Keulemans, W. Broothaerts, W.F. Broekaert |
| Keywords: | Malus pumila, pollination, S gene, S ribonuclease |
| Abstract:
We developed a molecular technique for the diagnostic identification of 11 different self-incompatibility alleles (S alleles) in apple. The method consists of allele-specific PCR amplification of genomic DNA. Different applications of our S allele typing method are described. The S allele-specific PCR analysis was applied to leaves, seed embryos and in vitro shoots of apple. DNA templates used in the analysis are prepared either by a single-step NaOH extraction method or by a longer CTAB-miniprep method. | |
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