ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
CHARACTERISATION OF ISOZYME VARIABILITY OF PEARS (PYRUS COMMUNIS L.) AND QUINCE (CYDONIA OBLONGA MILL.) IN VARIOUS TISSUES |
| Authors: | M. Hudina, F. Štampar, M. Viršcek-Marn, J. Smole |
| Keywords: | cultivars, rootstocks, graft, cultivar identification, incompatibility |
| Abstract:
Esterase (EST) and malate dehydrogenase (MDH) isozyme systems of eight cultivars of pear (Pyrus communis L.) and two rootstocks (Cydonia oblonga Mill.) were analysed by polyacrylamide gel electrophoresis. Leaves, vegetative buds and generative buds were used for extraction. The best results were obtained with isozymes extracted from buds. High variability of EST and MDH isozymes was established among different tissues of individual cultivars. Sufficient variability was detected for differentiation of all studied cultivars, including ‘Bartlett’ and its red sport ‘Red Bartlett’. EST proved to be the most favourable enzyme for the identification of pear cultivars. An electrophoretic band of EST from leaves and from vegetative buds was found to be common to rootstocks (Quince A and BA 29) and cultivars used as interstocks (‘Beurré Hardy’ and ‘Vicar of Winkfield’) and may be associated with the compatibility of rootstocks and pear cultivars. | |
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