ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
RAPD ANALYSIS OF HYBRID SEEDLINGS FROM A WILD APOMICTIC APPLE PARENTAL LINE |
| Authors: | Y. Koga-Ban, T. Kudo, M. Ishiyama, M. Suzuki, T. Kon |
| Keywords: | Malus transitoria, Malus hupehensis, rootstock, dwarf |
| Abstract:
To breed new apomictic, dwarf apple rootstocks, we made crosses of apomictic seed parents and dwarf pollen parents. The former included the species Malus transitoria and M. hupehensis, and the latter the dwarf rootstocks, M.9, M.26, M.27 and Bud. 9. We obtained about 1,500 seedlings and, to determine the nature of these seedlings, we applied RAPD analysis to distinguish between apomictic and hybrid forms. From 329 seedlings from the cross between M. transitoria and M.27, we were able to identify 40 hybrid seedlings by morphological observations, but 58 hybrid seedlings by using RAPD analysis; 38 seedlings were defined as hybrids by both morphological and RAPD analysis. We also performed RAPD analysis on 503 seedlings from the other crosses. These data demonstrate that RAPD markers can be very useful for identifying hybrids effectively and quickly. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
