ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
CHARACTERIZATION OF THE ‘BRACHETTO’ GRAPEVINE CULTIVARS USING MOLECULAR MARKERS |
| Authors: | R. Botta, R. Vallania, A. Akkak, M.L. Miaja, G. Vergano, M.R. Thomas, N.S. Scott |
| Keywords: | DNA, microsatellite, STMS, RAPD, isoenzymes, Vitis vinifera |
| Abstract:
Under the name of ‘Brachetto’ different grapevine cultivars are grown in Piemonte, in North-west Italy. Four cultivars have been described so far, all of them yielding black aromatic grapes: ‘Brachetto di Montabone’, ‘Brachetto di Nizza Monferrato’, ‘Brachetto del Roero’ and ‘Brachetto d'Acqui’. Only the last is officially authorised for planting and for the production of the corresponding DOC wine, “Brachetto d'Acqui”, which is an aromatic semi-sparkling wine in great demand. PGM and GPI isoenzymes and RAPD and STMS microsatellite markers were studied in the four known varieties and their clones. The combined profiles obtained with the two enzyme systems were able to distinguish the four cultivars but not the clones. The same results were obtained by the DNA typing methods which showed good polymorphism within the group of varieties. | |
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