ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
A LINKAGE MAP OF ISOENZYME LOCI IN THE CHERRY PROGENY PRUNUS AVIUM ‘NAPOLEON’ X P. NIPPONICA |
| Authors: | R. Boškovic, K.R. Tobutt |
| Abstract:
We are developing a genetic map of cherry, and have analysed Prunus avium ‘Napoleon’, P. nipponica and 47 seedlings from the interspecific cross for enzyme markers. Proteins extracted, usually from young leaves and from bark, were separated using polyacrylamide-gel electrophoresis or, occasionally, isoelectric focusing. Gels were stained for 46 different enzyme systems of which 28 proved to have at least one segregating locus. After genetic interpretation of the banding patterns, segregations and cosegregations were analysed using LINKEM software. By inspection of linkages with recombinations not exceeding 25% a linkage map was constructed. It comprises 10 linkage groups containing 49 isoenzyme genes at 41 points. It covers ~ 336 recombination units. These markers are ‘real’ genes showing allelic variation, unlike RFLPs and RAPDs on DNA maps. A further 13 isoenzyme genes are still unmapped. Some of the linkages are similar to those reported in e.g. almond and in apple. The map is providing a framework for DNA markers which are being added as part of a collaborative European project on Prunus mapping. | |
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