ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
THE USE OF INTERSPECIFIC CROSSES IN MALUS TO MAP THE GENES OF CHARACTERS IMPORTANT FOR APPLE ROOTSTOCK BREEDING |
| Authors: | Igor V. Bartish, Norman F. Weeden |
| Keywords: | Malus robusta, woolly apple aphid, Eriosoma lanigerum, isoenzymes, RAPD |
| Abstract:
With a view to generating a linkage map, reciprocal interspecific crosses between Malus Ottawa 3 clonal rootstock and M. robusta, segregating for resistances to fireblight (Erwinia amylovora), woolly apple aphid (E. lanigerum) and powdery mildew (Podosphaera leucotricha), as well as for suckering and burrknots, were analysed for isoenzyme and RAPD markers. For isoenzymes, 34 segregating loci were found in 14 polymorphic systems. Six more systems tested gave either no activity or no polymorphism. For RAPD markers, 72 decameric primers of 200 tested produced more than 400 polymorphic fragments. The segregation ratio for many of the RAPDs appeared to be dependent on the direction of the cross. The incidence of distorted segregation was about five times greater in the Ottawa 3 x Robusta 5 cross, than in the reciprocal (25% and 5% respectively). | |
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