ISHS Acta Horticulturae 484: Eucarpia Symposium on Fruit Breeding and Genetics
SELECTION OF SWEET CHERRY SEEDLINGS HOMOZYGOUS FOR SELF-COMPATIBILITY |
| Authors: | R. Boškovic, K.R. Tobutt, K. Russell |
| Keywords: | genetics, Prunus avium, ribonuclease |
| Abstract:
The self-compatible sweet cherry cultivar ‘Stella’ is known to have the genotype S3S4' where S4' is a self-compatible mutation of S4' such that S4' pollen can grow on S4 styles. We have analysed a progeny from selfing ‘Stella’, for stylar ribonucleases, which we have shown previously to correlate with S alleles. For each seedling, protein was extracted from about 20 styles, separated electrophoretically using isoelectric focusing and stained for ribonucleases. We detected two patterns, segregating approximately 1:1, one with two bands like ‘Stella’ and the other with a single band. We interpret these as S3S4' and S4'S4' respectively. This accords with the prediction that the S4' pollen, but not the S3 pollen, succeeds on the self styles. Two S4'S4' seedlings with large fruit have been selected, one with white flesh and one with black. If these are crossed with other cherries, all the seedlings should be self-compatible. Previously, the only way to raise wholly self-compatible progenies was to cross a self-compatible cultivar on to a cherry with the same incompatibility allele, i.e. in the case of ‘Stella’ on to a cherry with S3. | |
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