ISHS Acta Horticulturae 482: International Symposium on Cut Flowers in the Tropics
IN VITRO ADVENTIVE REGENERATION FROM CARNATION (DIANTHUS CARYOPHYLLUS) ANTHERS |
| Authors: | T. Mosquera, L.E. Rodríguez, A. Parra, M. Rodríguez |
| Keywords: | Haploids, gametic embryogenesis, microspore |
| Abstract:
Anthers obtained from flowers buds of carnation cv. 'Tanga' were used in order to establish a method for adventitious regeneration. A correlation between the microspore stage, anther length and the bud flower size was determined. It was found that anthers from 2,1–3,0 mm length have the highest levels of late uninucleated and early binucleated microspores. These anthers came from terminal flower buds, and their longitude was 1,0–2,0 cm. Greatest callus induction was obtained when the anthers were incubated on total dark conditions during five weeks on Murashige & Skoog (MS) basal medium supplemented with one mg/L of 2,4-Dichlorophenoxyacetic acid (2,4-D) and one mg/L of 6-Benzylaminopurine (BAP). Thirty embryogenic proembryos were obtained, and only one plantlet regenerated. The gibberellic acid (GA3) has not effect on the proembryo's development. | |
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