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ISHS Acta Horticulturae 481: International Symposium on Growing Media and Hydroponics

EFFECTS OF NUTRIENT AND ENVIRONMENTAL PRE-TREATMENTS ON THE OCCURRENCE OF ROOT INJURY OF LETTUCE CAUSED BY CHLORAMINE

Authors:   S. Date, T. Hataya, T. Namiki
Keywords:   Lactuca sativa L., chloramine, phytotoxicity, root injury, 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity, growth rate, nutrient element, environmental conditions
Abstract:
Lettuce seedlings pretreated with solutions containing either of N, P, K, Ca or Mg at the concentration of 150% strength Enshi-shohou for 48 hours, were transplanted to 50% strength Enshi-shohou solution containing chloramine. Root injury occurred by chloramine treatment with all the pretreatments. After 6 h of chloramine treatment, 2,3,5-triphenyltetrazolium chloride (TTC) reduction activity decreased in root tissues. Growth rate after 10 days of culture was similar with all the pretreatment. Lettuce seedlings subjected to high atmospheric temperature, high humidity, high solution temperature or high light intensity during culture with the half strength Enshi-shohou nutrient solutions for 8 days, were transplanted to 50% strength Enshi-shohou solution containing chloramine. Again, root injury occurred by chloramine treatment with all the pretreatments. TTC reduction of root tissues after 6 hours of chloramine treatment decreased similarly with all the pretreatments. Rate of fresh weight increase after 10 days of culture was similar with all the pretreatments. It was concluded that root injury was induced and growth was inhibited by chloramine, regardless of nutrient solution constituents or environmental conditions prior to chloramine treatment, although the effect of chloramine was modified by these pretreatments.

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