ISHS Acta Horticulturae 480: I International Symposium on Fig
ISOZYME AND RAPDs CHARACTERISATION OF A COLLECTION OF FIG (Ficus carica L.) TRADITIONAL VARIETIES |
| Authors: | P.J. Elisiário, M.C. Neto, L.F. Cabrita, J.M. Leitão |
| Keywords: | Isozymes, RAPDs, figs, DNA extraction |
| DOI: | 10.17660/ActaHortic.1998.480.25 |
| Abstract:
Isozyme and RAPD markers were used to distinguish among 55 traditional varieties of Ficus carica L. These varieties constitute the main Portuguese field collection of fig tree germplasm, which was implemented five years ago by the Direcção Regional de Agricultura do Algarve in Tavira, Algarve. Each variety is represented in the field by three to six plants with a total number of one hundred and eighty five young trees. During the first stage of our study all the plants were submitted to isozyme analysis. Six isozyme systems were revealed after starch gel electrophoresis of leaf extracts: PGI, PGM, IDH, MDH, GOT and LAP. Three isozyme systems, PGM, IDH and GOT revealed four polymorphic loci and were used for variety characterization. Errors of collection classification were detected. In one case, one out of three trees supposedly representing one clone showed clearly different isozyme patterns. In a second case, six plants presumably belonging to the same clone, were shown to represent two clones with three plants in both. Genetic similarities were calculated among all the remaining varieties and after clustering analysis a phenogram was elaborated according to UPGMA. Several clones shared identical isozyme patterns and could not be distinguished by these molecular markers. RAPDs were use in a second step of molecular characterization. Sixty decamer primers were tested. Forty-three primers generated amplified products and were used to distinguish among all the clones, either within groups of identical or different isozyme patterns. An easy and rapid method for genomic DNA extraction from fig leaf and bud tissues, used successfully in our lab for RAPD analysis of other fruit tree species, is described. | |
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