ISHS Acta Horticulturae 479: IX International Asparagus Symposium
CONSTRUCTION OF A LINKAGE MAP OF ASPARAGUS OFFICINALIS THROUGH DIFFERENT MOLECULAR MARKERS |
| Authors: | E. Caporali, P. Portaluppi, A. Spada, G. Marziani, A. Falavigna, F. Restivo, F. Tassi |
| Abstract:
Genetic maps are useful tools for plant breeding: they represent sources of markers to locate single or quantitative trait loci and can be used in marker-facilitated breeding programs. In a previous work we reported a linkage arrangement of RFLP loci in progenies from crosses between doubled haploid asparagus clones (Restivo et al., Theor. Appl. Genet. 90: 124–128, 1995) and more recently, a linkage map with 48 RFLP markers arranged in 14 groups was reported (Lewis and Sink. Genome 39: 622–627, 1996). However, a saturated map of asparagus is not yet available. In this work we present a linkage arrangement derived from the integration of the RFLP markers with two other molecular markers, RAPD and AFLP. The AFLP technique which has recently been adopted has already provided a high percent of polymorphism and several markers which have been integrated in the map. The construction of an asparagus map allows the identification of sex-linked markers; in the previous work we reported three RFLP markers of this kind; in this work we show two more RFLP and one more AFLP marker which segregates with sex. A saturated map of the sex chromosome could in the future allow the isolation of sex determining genes by positional cloning. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
