ISHS Acta Horticulturae 479: IX International Asparagus Symposium
DEVELOPMENT OF A MICROSPORE CULTURE METHOD TO PRODUCE HAPLOID AND DOUBLED-HAPLOID ASPARAGUS (ASPARAGUS OFFICINALIS L.) PLANTS |
| Authors: | M. Peng, D.J. Wolyn |
| Abstract:
The effects of genotype, cold pretreatment of flower buds and microspore isolation method were investigated for asparagus microspore culture. Binucleate microspores resulting from symmetric rather than asymmetric pollen mitosis I were observed after seven days cold treatment of flower buds and divided further in vitro after being shed from anthers. Significant correlations were found among the percentage of late-uninucleate microspores in the anther before cold treatment, the frequency of symmetric pollen mitosis after seven days of cold treatment and yield of microspore derived calli. Approximately 30% viable microspores were produced from two mechanical isolation methods, homogenization and microblending with viable microspore enrichment on Percoll gradients, while shed microspore culture generated more than 70% viable microspores. Calli were obtained only from shed microspore culture. Genotypes responded differently to shed microspore culture; responses for six genotypes were 144, 60, 56, 41, 4 and 0 calli produced per 100 anthers cultured. | |
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