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ISHS Acta Horticulturae 479: IX International Asparagus Symposium

OPTIMIZING THE DELIVERY OF DNA AND TRANSIENT GENE EXPRESSION IN ASPARAGUS CELLS BY BIOLISTIC BOMBARDMENT

Authors:   A. Chackalamannil, J. Eberhard, C. Laramore, F. Belanger, C. Chin, S. Garrison
Abstract:
The delivery of DNA by biolistic bombardment to asparagus cells and its expression were found to be affected by the rupture disc, microcarrier, osmotic potential, promoter of the gene, and the cell genotype. Significant improvement could by obtained by optimizing these factors.

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