ISHS Acta Horticulturae 479: IX International Asparagus Symposium
EARLY FLOWERING PROTEIN IN THE ARTIFICIAL FLOWERING PHASE IN ASPARAGUS SEEDLINGS |
| Authors: | T. Abe, D. Yeo, H. Abe, S. Yoshida |
| Keywords: | flowering, thiocarbamate, pathogensis related protein |
| Abstract:
Flower formation in one-month-old seedlings of Asparagus officinalis L. can be induced by soaking the seeds in thiocarbamate solution. We surveyed the protein profiles in the treated seedlings by 2-D PAGE, and found a new protein (EFP: Early Flowering Protein) accumulated during the chemical treatment. The cloned EFP cDNA consists of 719bp with a 480bp ORF which encodes 150 amino acids. The deduced amino acid sequence of EFP shows partial homology to the predicted amino acid of AoPRI (57% identity). The level of EFP in seedlings increased markedly during 4 days with the thiocarbamate treatment. Flowering activity of thiocarbamates corresponded with an increased level of EFP. We also investigated developmental changes in the level of EFP in seedlings and adult plants. EFP expression was clearly shown in male flowers, although it was absect from the stem and cladophylls. A trace of EFP was recognized in roots and in the early developmental stage of female flowers. The significance of EFP in the male flowers suggests a functional role in the male floral development. | |
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