ISHS Acta Horticulturae 479: IX International Asparagus Symposium
DETECTION OF VARIATION WITHIN ASPARAGUS EMBRYOGENIC CALLI USING RAPD MARKERS |
| Authors: | W.O. Hollingsworth, C.B. Christie, M.A. Nichols, M.H. Behboudian |
| Keywords: | Asparagus officinalis, micropropagation, plant growth regulators RAPDs, somatic embryogenesis, somaclonal variation |
| Abstract:
The efficiency of random amplified polymorphic DNA (RAPD) techniques to amplify polymorphisms occurring during culture of asparagus (Asparagus officinalis L.) embryogenic calli maintained on plant growth regulator (PGR) medium over a one year period was investigated. DNA fragments generated by 10-base primers were separated on 8% polyacrylamide gels, and were silver stained. Both cultivars 'Aspiring' and 'Pacifica' used in this study were distinguished by unique banding patterns generated by each primer. The technique also detected changes in genomic DNA structure induced during culture of embryogenic calli. Primer OPC-01 revealed more genomic variation than the primers OPC-02, -07, -08 and -09. These results suggest that this primer may amplify genetically unstable hypervariable regions within the genome. No consistent effect of PGR or length of time of culture on banding pattern was observed for either cultivar investigated. This study shows that RAPD technology can be used to detect somatic variation occurring during long-term culture of asparagus embryogenic calli maintained on PGR medium. | |
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