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ISHS Acta Horticulturae 478: VI International Symposium on Plum and Prune Genetics, Breeding, Pomology

GENETIC RELATIONSHIPS IN JAPANESE PLUM CULTIVARS BY MOLECULAR MARKERS

Authors:   E. Bellini, E. Giordani, V. Nencetti, D. Paffetti
Keywords:   Prunus, Japanese plum, molecular marker, RAPD
Abstract:
The plum group appears to have the greatest genetic diversity respect to several other subgenus belonging to Prunus genus. The term Japanese plum originally was applied to Prunus salicina Lindl. (formerly P. triflora Roxb.), but now encompasses a wide range of fresh-market plums developed by intercrossing various diploid species with other species. The capability to distinguish among plum cultivars in breeding and cultivation is extremely important. We investigated the genetic diversity among 31 genotypes of Prunus spp.: 20 of Japanese plum, 7 of P. domestica L., 1 of plumcot, 1 of P. cerasifera Ehrh., 1 of P. armeniaca L. and 1 of P. persica L.. Their genetic variability by RAPD (Random Amplified Polymorphic DNA) technique was investigated. Relationships among the 31 genotypes were represented by a dendrogram, constructed using the Euclidean distance matrix and the Neighbor-joining method. The RAPD markers were successfully used for identification of the genotypes. The unrooted dendrogram showed the plum cluster (P. salicina Lindl. and P. domestica L.) distincted from P. armeniaca L. and P. persica L

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