ISHS Acta Horticulturae 474: III International Symposium on Olive Growing
HISTOLOGICAL OBSERVATIONS ON SOMATIC EMBRYOS OF OLIVE (OLEA EUROPAEA L.) |
| Authors: | M. Lambardi, A. Caccavale, E. Rugini, G. Caricato |
| Keywords: | Olive, somatic embryos |
| Abstract:
A study on the ontogeny of secondary somatic embryogenesis in olive (cv. Canino) was carried out. In this system, morphogenetic masses developed from mature tissues (petioles of regenerated shoots), and originated primary embryos. By subculturing primary embryos, highly efficient continuous cycles of direct secondary embryogenesis were obtained. Histological observations showed early evidence of embryo primordia as densely-stained groups of 5 or more meristematic cells, located mainly in the epidermal layer of the primary embryo. These primordia evolved into globular embryos, that grew to the cotyledonary-stage through the heart-shape and the torpedo-shape stages. Secondary embryos seem to have a multicellular origin; however, a very limited number of cells of the primary explant is apparently involved in the formation of embryo primordia. A unicellular pathway is not to be disregarded either. | |
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