ISHS Acta Horticulturae 474: III International Symposium on Olive Growing
VARIETAL DISCRIMINATION OF 15 GENOTYPES OF OLIVE (OLEA EUROPAEA L.) BELONGING TO CV. MANZANILLA |
| Authors: | G. Vergari, M. Patumi, G. Fontanazza |
| Keywords: | Olive, varietal discrimination |
| Abstract:
It is difficult to identify and classify olive (Olea europaea L.) varieties, which are estimated as being close to 2,000 in number. Morphological and physiological traits are widely used for this purpose, but they are strongly dependent on the environmental factor, consequently it is difficult to the resolve the issues that arise concerning synonymy and homonymy. In order to conduct a careful study of the genetic diversity within the olive germplasm and to highlight varietal polymorphism, 15 genotypes of O. europaea belonging to the Manzanilla cultivar were examined. They are different in terms of morphological traits and were collected from Spain (10), Argentina (2), Portugal (1), Israel (1) and the United States (1). The RAPD (Random Amplified Polymorphic DNA) technique was used to distinguish these genotypes. Genetic analysis was carried out on genomic DNA extracted from leaves. For DNA amplification, two Operon kits (OP-A and OP-B) were used, each of them consisting of 20 random-sequence decamers. Statistical analysis was carried out by means of the NTSYS program in order to verify the genetic distance among the genotypes studied. All the primers produced polymorphic bands, which were used to costruct a genetic similarity matrix. | |
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