ISHS Acta Horticulturae 474: III International Symposium on Olive Growing
OLIVE BIODIVERSITY DETECTED BY AFLP MARKERS |
| Authors: | A. Angiolillo, L. Baldoni |
| Keywords: | Olive, biodiversity, AFLP markers, cluster analysis |
| Abstract:
The phylogenetic relations among cultivated olive (Olea europaea L.) and others Olea species are still under investigation and only some hypotheses on the origin of cultivars and on their derivation from ancestral species and wild genotypes were formulated. The aim of the work was to carry out a comprehensive study on the biodiversity inside the Olea genus and to monitor, in the wild germplasm, the presence of agronomically interesting traits, such as pest and insect resistance or tolerance to abiotic stresses. The screening has considered varieties widely cultivated in the mediterranean area in comparison with some Olea species. Amplified Fragment Length Polymorphism (AFLP) markers were used because of their ability to generate a large number of polymorphisms in a fast and reproducible manner. Four different primer combinations were used, showing a high level of polymorphic bands. The obtained data were elaborated to measure the genetic similarity and, after cluster analysis, the resulting dendrogram is shown and discussed. AFLP markers proved to be a reliable and efficient technique to evidence polymorphism among the genotypes under investigation and hence can be considered a powerful tool to determine genetic relations. | |
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