ISHS Acta Horticulturae 474: III International Symposium on Olive Growing
OLIVE EMBRYO DEVELOPMENT STAGE AND THE POSSIBILITY OF OBTAINING VIABLE SEEDLINGS |
| Authors: | J. Liñán, A. Troncoso, H.F. Rapoport |
| Keywords: | olive, embryo development |
| Abstract:
When 'Manzanillo' olive embryos were excised at successive dates and cultured on one-third strength Murashige-Skoog medium, three stages of differing growth potential were observed: 1) An initial stage in which no germination was obtained, 2) An intermediate 'critical' period in which germination and viability percentage increased according to date and 3) A final stage, from the time when the embryo reached full size, in which germination and viability were maximum. In the intermediate 'critical' period the seed had obtained its final length and the embryo was undergoing rapid expansive growth. Anatomical studies indicated the presence of the future root and shoot apices and well-defined provascular strands throughout this period. As time progressed, however, the embryo tissues seemed to acquire greater metabolic content and cytoplasmic integrity. | |
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