ISHS Acta Horticulturae 474: III International Symposium on Olive Growing
THE BUFFERING EFFECT OF OLIVE EMBRYOS ON THE PH OF THE SUBSTRATE, DURING THEIR IN VITRO GERMINATION |
| Authors: | T.S. Pritsa, D.G. Voyiatzis, T.A. Matsi |
| Keywords: | Olive embryos, in vitro culture, germination, pH, soluble salts |
| Abstract:
Olive embryos germinating in vitro, on a solidified aqueous substrate without nutrients, caused a change in its pH, which had been initially adjusted at 4 or 7. In both media (pH 4 and pH 7), the pH increased until the germination started and then decreased. In all cases the final pH, after 40–60 days, had been adjusted to around 5. The changes of the pH of the medium appeared to follow the various phases of germination, indicating the possible involvement of chemical factors released by the embryos. Quantitative determination of inorganic ions in samples of the culture medium, at regular time intervals, revealed changes in their concentration, denoting their movement into- and out of the explant tissues. These changes were made prominent after germination had started and the pH of the medium started to decrease and coincided with the phase of cell elongation and radicle emergence. The pattern of these changes indicated that from day 20 onwards, cell division starts to accelerate. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
