ISHS Acta Horticulturae 473: International Symposium on the Importance of Varieties and Clones in the production of Quality Wine
GENETIC MARKERS FOR THE IDENTIFICATION OF VARIETIES AND CLONES AS A GUARANTEE OF QUALITY |
| Authors: | F. Regner, K. Sefc, A. Stadlbauer, H. Steinkellner |
| Keywords: | Grapevine, Vitis, wine quality, vine, clones, DNA profiling, DNA markers, identification, Simple Sequence Repeat, Random Amplified Polymorphism Detection |
| Abstract:
Several different marker systems were applied to label grapevine varieties and clones. Identification of cultivars and association to defined clones was rendered possible by developing SSR, RAPD and AFLP markers. Using SSR markers identification of cultivars was feasible with the exception of colour types of one cultivar like the Pinot family. 220 cultivars could be differentiated. Otherwise RAPD and AFLP markers fulfil the demands for differentiating clones or biotypes of one cultivar. Besides the identification of the cultivars the characterization of allel length at more than 30 SSR loci enabled us to define the relationship of the cultivars. For some varieties (Cabernet Sauvignon, Silvaner, Rotgipfler, Frühroter Veltliner and Neuburger) the definition of their origin could be proposed for the first time. For the cultivars Müller Thurgau and Jubiläumsrebe the definition of their heritage has to be corrected. Furthermore RAPD and AFLP markers were used to differentiate clones of Grüner Veltliner and of Chardonnay as well as Pinot blanc. These genetic markers could be used to identify varieties and clones and therefore will give a guarantee to the growers to plant the right genotypes. Furthermore SSR markers were used to control the hybridization in cross breeding steps. In populations usually less than 5% selfings could be detected with one exception, where nearly 50% of the vines missed hybridization. | |
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