ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
VIRUS-FREE PLUM AND PEACH MOTHER PLANT PRODUCTION IN EGYPT |
| Authors: | M.A.E. Awad, L.M. Ibrahem, A.E. Aboul-Ata, M. Ziedan, H.M. Mazyad, E. Abdel-Aziz, N. Mansour |
| Abstract:
The main criteria for selecting plum and peach mother trees include fruit yield (Kg / tree), volume, weight, color, and seed (free seed or cling) total soluble sugar content, canopy surface area, age (not less than 7 years), and healthy appearance (free of viral and fungal symptoms, insect infestation and nutrient deficiency). Selected mother trees were marked and maps were drawn for the tree sites at each location. Four leaf samples were collected from four different sides of the tree. Virus detection was performed using DAS-ELISA and TBIA techniques. Polyclonal antibodies against plum pox, prune dwarf virus, prunus necrotic ring spot virus, peach rosette mosaic virus, arabis mosaic virus and tomato ring spot virus were used. One plum orchard (or site) at Giza, 3 at Qualubia and 2 at Munofia were selected. Fifty-nine percent of 34 tested trees at Giza, 66% of 80 trees at Qualubia, 100% of 10 trees at one site in Munofia and 57% of 1311 trees at another site in Munofia were found healthy (virus-free). One peach site at Giza, 3 at Qualubia, 1 in Munofia, 3 in Dakahlia, 1 in Behaira and 4 in north Sinai were selected. One-hundred percent of 2 tested trees in Giza, 77% of 22 trees in Qualubia, 100% of 10 trees in Munofia, 60% of 20 trees in Dakahlia, 67% of 3 trees in Behaira, and 100% of 10 trees in North Sinai were healthy (virus-free). Budwood from virus-free plum and peach mother trees were grafted on one year old plum and peach seedlings, respectively. Grafted seedlings have been kept in a greenhouse under controlled temperature, humidity, light and ventilation for virus-free mother plant repository performance. | |
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