ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
MOLECULAR EVIDENCE FOR THE OCCURRENCE OF PLUM POX VIRUS - CHERRY SUBGROUP IN HUNGARY |
| Authors: | L. Nemchinov, A. Hadidi, M. Kölber, M. Németh |
| Abstract:
Leaf samples from peach GF 305 seedlings graft-inoculated with bud chips from sweet cherry trees cv. ‘Van’, which tested positive for plum pox virus (PPV) by ELISA, were analyzed by molecular methods for the presence or absence of PPV-Cherry (PPV-C). These analyses indicated that the sweet cherry cultivar is infected with a member of the established PPV-C subgroup as shown by RT-PCR amplification of PPV cDNA of the expected size using DNA primers specific for PPV-C and the demonstration that nucleotide sequence analysis of the cloned RT-PCR product contains the distinct 5'-terminal sequence of the coat protein coding region, characteristic for PPV-C members. Our results also suggest that PPV-C may be more widespread in eastern Europe than was originally thought. | |
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