ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
A FIVE-YEAR STUDY TO DETERMINE THE EVENTUAL OCCURRENCE OF PLUM POX VIRUS IN CHERRY CULTIVARS IN HUNGARY |
| Authors: | M. Kölber, M. Németh, G. Tõkés, L. Krizbai, S. Szõnyegi, I. Ember, Zs. Bereczky, E. Pocsai, R. Hangyál, Á. Vollent, E. Bencze, E. Papp, A. Pete, Gy. Hajnóczy, E. Kiss, P. Imre, M. Takács, F. Merõ |
| Keywords: | ELISA, RT-PCR, biological test, sweet and sour cherry cultivars, cherry rootstocks |
| Abstract:
A five-year study was carried in the 1990's to determine the eventual occurrence of PPV in cherry cultivars in Hungary. Surveys were made by DAS-ELISA and biotin-avidin ELISA. Selected ELISA-positive trees were further tested with indexing on woody and herbaceous indicators as well as by ELISA and RT-PCR. Between 1992–1996, 403 trees reacted positively in ELISA out of more than 9000 trees that belong to 7 sour cherry and 19 sweet cherry cultivars. Infected trees were found also in the three rootstock species tested. Slight leaf symptoms occurred only on two out of 59 ELISA-positive trees until now. Field indexing of 19 ELISA-positive trees was negative, none of the GF 305 and GF 31 peach indicators showed symptoms. Under greenhouse conditions, GF 305, Nicotiana benthamiana and N. clevelandii plants produced slight symptoms when inoculated with PPV from the sweet cherry cultivar ‘Van’ grown in the southern part of Transdanubia. PPV was detected by ELISA and RT-PCR in these indicators. Cloning and nucleotide sequencing of this 'PPV-Van' isolate demonstrated that it is a member of the PPV-Cherry subgroup (Nemchimov et al., 1998). Mechanical transmissions from 5 other sweet cherry cultivars gave similar results on Nicotiana plants to those of the ‘PPV-Van’ isolate. RT-PCR detected the presence of PPV in 14 out of 17 cherry trees that belong to 11 cherry cultivars, and in two rootstock species. Further experiments are needed to check the ELISA-positive trees with PCR using strain-specific primers and to continue biological characterization of the Hungarian PPV-cherry isolates. | |
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