ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
DETECTION OF PLUM POX VIRUS IN ROOT AND BARK SAMPLES FROM DORMANT PLUM TREES |
| Authors: | A.N. Adams, C.M. Guise, S.J. Crossley |
| Keywords: | ELISA, polymerase chain reaction, PCR, nursery, Prunus |
| Abstract:
An immuno-capture polymerase chain reaction (IC-PCR) protocol and ELISA were compared for their effectiveness in detecting plum pox virus (PPV) in dormant plum material. Although the IC-PCR was about 1000 times more sensitive than ELISA, PPV was detected by ELISA in 71–80% of bark samples collected in December, January and March from pot-grown rootstock trees inoculated with PPV the previous March, compared to 85–86% detection in the same samples by IC-PCR. In similar samples from one year old shoots taken from infected branches of orchard trees, 66–81% were positive by ELISA compared to 81–87% by IC-PCR. With bulked samples taken from the fibrous roots of the pot-grown trees, PPV was detected in 92–100% of samples by IC-PCR in winter compared to only 38–65% by ELISA; the extra sensitivity of IC-PCR may offer a reliable method for assessing infection in young trees and rootstocks lifted from nursery rows. | |
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