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ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops

DETECTION AND SEROLOGICAL IDENTIFICATION OF PLUM POX VIRUS ISOLATES IN THE CZECH REPUBLIC

Authors:   M. Navrátil, V. Šimonová, F. Paprštein, R. Karešová
Keywords:   plum pox virus, monoclonal antibodies, serotypes, detection, ELISA
Abstract:
Plum pox virus is considered one of the most damaging virus diseases of plums, apricots and peaches in the Czech Republic. Seventy-three plum pox virus isolates from different growing areas and hosts were characterized using a set of monoclonal antibodies, including D specific, M specific and universal. Fifty-two isolates belong to PPV-D serotype, 20 isolates were recognized by M specific monoclonal antibody and one sample of mixed infection was detected. Distribution of PPV-D and PPV-M serotypes in the Czech Republic indicated original and/or massive spread of PPV-D isolates in the Czech Republic.

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