ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
DETECTION OF CHERRY VIRUS A IN CANADA AND GERMANY |
| Authors: | D. James, W. Jelkmann |
| Abstract:
Cherry capillovirus A (CVA) has recently been identified as a new virus in sweet cherry. The virus was detected in sweet cherry sources of different disease status but CVA could not be related to any of the currently described diseases in sweet cherry. Sensitive detection tests, based on RT-PCR and on digoxigenin-labeled cDNA probes, were developed to initiate a survey for CVA. In RT-PCR analysis CVA specific primers were used that amplified a 834 bp product. Samples of Bing, Sam, Canindex and Prunus avium F12/1 infected with little cherry disease, cherry mottle leaf, rusty mottle, necrotic rusty mottle, green ring mottle, prunus necrotic ringspot, X-disease, and an agent causing stunting of Prunus serrulata 'Shirofugen' in various combinations were used as source material. CVA was detected by dot blot hybridization using the cDNA probes and by RT-PCR in nearly 40% of the samples investigated. CVA was also detected in apricot and peach. The healthy controls used in the study consistently gave negative results. The findings indicate a wide distribution of the virus in sweet cherry sources from Germany and Canada. However, the data do not allow the establishment of a relationship of CVA to any of the described diseases in sweet cherry. | |
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