ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
IDENTIFICATION OF A FLEXUOUS VIRUS ASSOCIATED WITH PEACH MOSAIC DISEASE |
| Authors: | D. James, W.E. Howell |
| Abstract:
A flexuous virus, peach mosaic virus (PMV), was isolated from a peach mosaic affected Elberta tree. The virus was mechanically sap-transmitted from symptomatic Elberta leaves to Chenopodium quinoa using 0.03 M potassium phosphate buffer containing 0.02 M mercaptoethanol and 1% polyvinylpyrrolidone (Mr =10,000). The virus has an estimated size of 752 nm in length, 9.3 nm in width, and with striations 3.5 nm in pitch. The herbaceous host range of the virus includes C. amaranticolor, C. quinoa, and Nicotiana occidentalis. A polyclonal antibody (PAb) was produced and used in ELISA and Western blot analysis to detect the virus in woody and herbaceous hosts. PMV has a coat protein subunit size estimated at 20.5 kDa. In assays to determine specificity, the PMV-associated PAb cross-reacted with cherry mottle leaf virus (ChMLV). Also, PAbs and monoclonal antibodies produced against ChMLV cross-reacted with PMV. The virus was transmitted to Elberta peach, by budding, and induced symptoms typical for peach mosaic. The presence of the virus was confirmed by ELISA and Western blotting. Bing cherry and Mahaleb seedlings do not appear to be hosts for PMV based on the results of serological analyses. | |
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