ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
DETECTION OF VIRUSES OF WOODY HOST PLANTS USING COLORIMETRIC PCR |
| Authors: | A. Rowhani, L. Biardi, D.A. Golino |
| Abstract:
A new colorimetric technique has been tested that does not rely on gel electrophoresis or molecular hybridization to detect virus-specific PCR products. This colorimetric method for detection of PCR products from woody plants was demonstrated to be at least as sensitive as gel analysis. When combined with immunocapture of virions from plant sap, colorimetric detection provides a means to apply PCR technology to a large number of samples. Here, we report on the use of this technique for detection and quantitation of a walnut isolate of cherry leafroll virus, citrus tristeza virus, prune dwarf virus, prunus necrotic ringspot virus, and tomato ringspot virus in woody and herbaceous plants. | |
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