ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
STRAIN-SPECIFIC POLYMERASE CHAIN REACTION ASSAYS FOR DISCRIMINATION OF PRUNUS NECROTIC RINGSPOT VIRUS ISOLATES |
| Authors: | R.W. Hammond, J.M. Crosslin, W.E. Howell, G.I. Mink |
| Keywords: | cherry, PNRSV |
| Abstract:
Nucleotide sequence alignment of a 1.65 kb PCR product obtained from RNA3 of several biologically distinct PNRSV sweet cherry isolates revealed correlations between symptom type and the nucleotide and amino acid sequences of the 3a (putative movement protein) and 3b (coat protein) open reading frames (Hammond and Crosslin, 1998). Based upon this analysis, reverse transcription-polymerase chain reaction (RT-PCR) assays have been developed that can distinguish between symptom types. The results of PCR amplification using type-specific primers that amplify a portion of the coat protein gene show that the primer-selection procedure developed for PNRSV constitutes a reliable method of viral strain discrimination in cherry. | |
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