ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
CHARACTERIZATION OF APPLE CHLOROTIC LEAF SPOT VIRUS ISOLATES FROM ITALY |
| Authors: | G. Pasquini, F. Faggioli, M. Pilotti, V. Lumia, M. Barba |
| Abstract:
Apple chlorotic leaf spot trichovirus (ACLSV) is a flexuous virus with a 720 x 12 nm particles which contain a single stranded RNA. It induces serious diseases in pome and stone fruits worldwide. A high biological and molecular variability is known to occur among ACLSV isolates. To determine the ACLSV isolates present in Italy, field survey of several fruit orchards in Italy were performed. The presence of the virus was preliminary ascertained by ELISA and by chip budding on woody indicators. Protein preparations of all positive samples (from 66 plants) were separated on 15% SDS-PAGE then analyzed by Western blotting with a polyclonal antiserum specific to an Italian apple ACLSV isolate. On the basis of the electrophoretic mobility of the coat protein, which ranged between 22.7 and 19.7 kDa, ACLSV isolates from Italy were divided into three distinct groups. These groups also differ in their biological properties. Immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) technique, applied to three isolates, representative of each group, followed by RFLP analysis with four restriction enzymes (Rsa I, Alu I, Hind III and Hinc II) revealed two different patterns. These patterns were also confirmed by nucleotide sequencing of the amplified products. | |
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