ISHS Acta Horticulturae 472: XVII International Symposium Virus and Virus-Like Diseases of Temperate Fruit Crops
APRICOT LATENT VIRUS: TRANSMISSION, PURIFICATION AND SEROLOGY |
| Authors: | E.Z. Zemtchik, T.D. Verderevskaya, Yu.A. Kalashian |
| Keywords: | apricot latent virus (ALV), test-plants |
| Abstract:
Apricot latent virus (ALV) was identified from symptomless apricot (variety Silistra N4) brought to Moldova from Bulgaria. Typical yellowish-green spots on leaves of peach seedlings appeared after chip-budding of infected apricot buds on healthy seedlings. Filamentous virus particles were detected by thin sectioning in the cytoplasm of parenchyma cells of infected peach leaves. The virus was mechanically transmitted from peach leaves to Nicotiana occidentalis B37 where it caused vein clearing of growing leaves and developed necrosis on inoculated leaves of N. occidentalis ssp obliqua N1. An antiserum to ALV with a titer of 1:512 was prepared. This antiserum and an antiserum to apple stem pitting virus cross reacted in ELISA and ISEM assays with extracts from ALV-infected peach seedlings. | |
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