ISHS Acta Horticulturae 470: II International Symposium on Pistachios and Almonds
SOMATIC EMBRYOGENESIS IN PISTACHIO (PISTACIA VERA L.) CV. MATEUR |
| Authors: | A. Chatibi, M.L. Kchouk, S. Thaminy, A. Ghorbel |
| Keywords: | 2,4D, kinetin, micropropagation, conversion, adventitious shoots, callogenesis, rhizogenesis |
| Abstract:
Despite recent success in the in vitro production of pistachio plants, several problems are still encountered in micropropagation, such as red pigmentation of leaves and bud necrosis. Therefore, the use somatic embryos could be an alternative that may overcome rooting problems and other obstacles in pistachio vitroculture. The development of embryos from leaves and internodes of apices taken from mature trees would give an excellent opportunity to propagate true-to-type cultivars. The present work reports on the induction of somatic embryogenesis of cotyledons, leaves and apical internodes of Pistacia vera, cv. Mateur. Results showed that the induction of callogenesis responds similarly in all types of explants, but the inducing medium for the development of somatic embryos differed with the nature of the explant. Somatic embryos were obtained on (i) 60% of cotyledons in presence of 1.0 mg.l-1 2,4D and 1.0 mg.l-1 kinetine, (ii) 63% of leaves in 2.0 mg.l-1 2,4D and 1.5 mg.l-1 kinetine and (iii) 68% of internodes in 5.0 mg.l-1 2,4D and 2.0 mg.l-1 kinetine. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
