ISHS Acta Horticulturae 470: II International Symposium on Pistachios and Almonds
MULTIVARIATE ANALYSIS OF THE ROLE OF INORGANIC ELEMENTS ON MICROPROPAGATION OF PISTACHIO (PISTACIA VERA L.) CV. MATEUR |
| Authors: | A. Chatibi, M.L. Kchouk, S. Thaminy, A. Ghorbel, T. Khsib |
| Keywords: | macroelement, microelement, red pigmentation, ions, apices, shoots, rooting |
| Abstract:
Micropropagation of pistachio (Pistacia vera L.) cv. Mateur has been initiated from apices and cuttings to obtain hundreds of rooted plantlets (Chatibi et al., 1995). However, some physiological disorders were reported. Beside bud necrosis, which could be prevented by addition of NAA and thiamine, a red pigmentation was often observed on leaves during multiplication and threatened acclimatization of plantlets even when rooting was not affected. It was noted that the addition of potassium (KCl) and aluminum chloride (AlCl3) in the medium limited the phenomenon, which implicates inorganic elements in the control of the disease. In the present work, we studied the effect of inorganic nutrients on the behaviour of adventitious shoots during multiplication with special reference to the red pigmentation of leaves and shoot vigor. Six macroelements and nine microelements have been investigated and Principal Component Analysis was used to view the experiment and screen the appropriate elements and concentrations for optimal micropropagation. Several media could eliminate red pigmentation of leaves. Low concentrations of macroelements KCl (at MS/2) and KH2PO4 (at 0.0 mg.l-1), and microelements H3BO3 (at MS/4, MS/5 and 0.0 mg.l-1), and KI (at MS/2 and MS/5) gave suitable conditions for total elimination of red pigmentation. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
