ISHS Acta Horticulturae 467: III International Symposium Diversification of Vegetable Crops
EVALUATION OF INTRASPECIFIC VARIATIONS OF ALLIUM VICTORIALIS VAR. PLATYPHYLLUM USING AMPLIFIED POLYMORPHIC DNA MARKERS |
| Authors: | H.T. Lim, Y.N. Song, K.U. You, W.B. Kim |
| Keywords: | Allium victorialis var. platyphyllum, PCR, intraspecific variation, habitat, M13 core sequence, random primers |
| Abstract:
The intraspecific variations among Allium victorialis var. platyphyllum populations from three habitats and among 41 individuals were investigated based on the amplified DNA polymorphisms derived from PCR (polymerase chain reaction) method. Eleven out of 81 primers (80 random [10-mer] primers, one 15-mer[M13 core sequence] primer) screened, resulted in DNA polymorphisms (61.5%) in all of the tested plants, producing 83 randomly and specifically amplified DNA fragments. The Ulleungdo population of Allium victorialis var. platyphyllum was differentiated from the two other populations at the 0.77 of similarity index value of DNA polymorphisms. However, the Jirisan and Odaesan populations were not differentiated from each other. As for the intraspecific variations of each population, the Ulleungdo population showed lower level of variations having higher similarity index of 0.92 compared with those of Jirisan and Odaesan populations that ranged from 0.86 to 0.87. | |
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