| Keywords: | Stachys sieboldii, flowering, floral biology, pollen viability, pollen germination, seed germination, RAPD, genetic variability, molecular characterization |
Abstract:
In order to increase the germplasm collection of Chinese artichoke (Stachys sieboldii) in the Department of Vegetable and Seed Crops of INH, and possibly to develop a breeding program for this species, seed tubers of a Chinese clone, coming directly from NERCV, Beijing, were planted in April 1989 at INH.
Unlike the French clone and the Chinese one in Beijing, all the plants issued from these tubers flowered and formed zygotic seeds during the first year of growth in France.
From these seeds, nine plants were obtained and four of them were cloned with success.
The physiological behaviour of the original Chinese clone and the four clones issued from seeds - named C1, C2, C3 and C4 - annually cultivated from seed tubers, were studied until 1996. At the same time, morphological and genetic characterization of the new clones were analysed.
During the seven observed generations, the original Chinese clone progressively lost its ability to flower and, since 1995, no flowering has been observed.
On the other hand, at each generation, only two of the new clones (C2 and C3) produced flowers; these produced virtually no seeds.
Morphological and molecular characterization of the four new clones and their comparison with the INH clone and the original Chinese clone are discussed.
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