ISHS Acta Horticulturae 465: IV International Peach Symposium
GENETIC ANALYSIS OF AGRONOMIC TRAITS AND GENETIC LINKAGE MAPPING IN A BC1 PEACH POPULATION USING RFLPS AND RAPDS |
| Authors: | R. Quarta, M.T. Dettori, I. Verde, A. Gentile, Z. Broda |
| Keywords: | Prunus persica, linkage map, RFLP, RAPD, powdery mildew |
| Abstract:
A progeny of 76 BCI seedlings, resulting from a cross between the semidwarf selection IF7310828 as female recurrent parent and a seedling of Prunus ferganensis used as donor, carrying a potential source of resistance to powdery mildew, was studied in order to construct a genetic linkage map of peach nuclear genome. Thirty-seven RFLPs, detected by genomic and cDNA clones, 3 morphological traits and 8 RAPDs were analyzed for inheritance and linkage. Two of the cDNA clones correspond to known genes (phosphoglyceromutase and peptide induced by jasmonic acid). Thirty-three loci segregated in a 1:1 ratio (28 were heterozygous in the F1 parent and 5 in the recurrent parent), 8 segregated in a 1:2:1 ratio and 7 in a 3:1 ratio. Nine loci showed a distorted segregation ratio. A genetic linkage map was elaborated with a total of 36 loci using JoinMap. Eight linkage groups covering 257 cM of the peach genome were found. Twelve loci remained unlinked. Linkage between leaf glands, Sphaerotheca pannosa resistance, flesh adhesion and molecular markers were found. | |
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