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| Authors: | H. Kato-Noguchi, Alley E. Watada, L. Qi |
Abstract:
The RQ breakpoint of carrot shreds occurred at about 1% O2. At slightly below the RQ breakpoint, 0.5% O2, glycolysis was affected.
The low O2 atmosphere caused a sharp increase in fructose 1,6-bisphosphate (Fru-1,6-P2) and a slight decrease in fructose 6-phosphate (Fru-6-P), phosphoenolpyruvate, and pyruvate among the glycolytic intermediates.
The low O2 also caused an increase in fructose 2,6-bisphosphate (Fru-2,6-P2) and ethanol.
Fru-2,6-P2 appeared to have promoted glycolysis, as noted by the increased activity of phosphate-dependent fructokinase.
ATP level decreased under low O2, but a sufficient level is thought to have been maintained for minimum metabolism by the increased glycolytic activity.
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