ISHS Acta Horticulturae 463: VIII International Symposium on Plant Bioregulation in Fruit Production
FEMINIZATION OF ANDROECIOUS PAPAYA LEADING TO FRUIT SET BY ETHREL AND CHLORFLURENOL |
| Author: | A. Kumar |
| Keywords: | Sex-reversal, free soluble proteins, peroxidase isozymes, disc gel electrophoresis |
| Abstract:
Exogenous application of ethrel (2-Chloroethylphosphonic acid) at 240 to 960 ppm 10-6 and chlorflurenol (2-chloro-9-hydroxyflurene(9)carboxylic acid) at 20 to 80 ppm 10-6 induced female and intersexual flowers along with male flowers on genetically male plants of papaya (Carica papaya L. var. honey dew) grown in field conditions. The induced female and intersexual flowers developed into fruits later on. This phenotypic expression of sex reversal was found associated with qualitative and quantitative changes in free soluble proteins and peroxidase isozymes. Polyacrylamide disc gel electrophoresis of soluble proteins revealed 3 specific proteins in male vegetative tissue which were not detected in its female counterparts, whereas 4 other proteins recorded in females were missing in the former. In male flower buds, 7 new bands of proteins appeared with concomitant disappearance of the other two bands of its vegetative stage. Similarly in female flower buds, 5 protein bands not detected in its vegetative tissue were noticed with the disappearance of five other bands recorded in the latter. The peroxidase activity was higher in female flower buds than in its male counterparts and the vegetative tissues. At the same time, 3 new bands of peroxidase isozymes were noticed in male flower buds and one in female flower buds as compared to its vegetative tissues. However, one peroxidase isozyme band present in vegetative tissues of both the sexes disappeared from the reproductive tissues. | |
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