ISHS Acta Horticulturae 462: I Balkan Symposium On Vegetables and Potatoes
COMPARATIVE INVESTIGATIONS OF SOME TOBACCO MOSAIC VIRUS ISOLATES FROM DIFFERENT HOST PLANTS |
| Authors: | B. Krstic, G. Stojanovic, I. Vico, M. Barac, B. Malinaric |
| Keywords: | tobacco mosaic virus, strains, pepper, sunflower, sugar - beet, bioassay, serology |
| Abstract:
Tobacco mosaic virus (TMV) isolates from pepper (isolate M5), sugar - beet (Bac and Kovin) and sunflower (Panašir, S1/94 and Opovo) were compared in these investigations. As standards, isolates Juretic (type strain isolated from tobacco), NŠ (chilly pepper necrotic strain), FO and P8 (strains isolated from pepper) were also included. Bioassay and serological methods compared investigated isolates and standards. Serological analyses were performed using agar double diffusion and electro - blot immunoassay (EBIA, Western blot). On the basis of the reaction of test plants and antigenicity, investigated isolates may be divided into two groups. The isolate M5 (from pepper), Kovin (sugar - beet), Panašir, S1/94 and Opovo (sunflower) are very closely related to the TMV type strain (isolate Juretic). The isolate Bac (from sugar - beet) differs from the type strain as well as from the other strains used in this study as standards (NŠ, FO and P8). Differences of Bac isolate as well as NŠ, FO and P8 obtained by bioassay were confirmed by serological analyses. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
