ISHS Acta Horticulturae 461: International Symposium on Biotechnology of Tropical and Subtropical Species Part 2
GUS GENE EXPRESSION IN ANTHURIUM ANDREANUM, ONCIDIUM GOWER RAMSEY AND BRASSOLAELIOCATTLEYA ORANGE GLORY EMPRESS AFTER PARTICLE BOMBARDMENT |
| Authors: | G. Chi, H. K.-L. Goh, K. Hoo, T. Legavre |
| Keywords: | Anthurium andreanum, Oncidium Gower Ramsey, Brassolaeliocattleya Orange Glory Empress, orchids, gene gun, biolistic technique |
| Abstract:
Different plasmids (pUGCI - maize ubiquitine promoter; pEF-1  - Arabidopsis thaliana promoter; and p70GUS - CaMV promoter) carrying the various promoters driving expression of the bacterial gene encoding for  -glucuronidase were coated onto gold microcarriers and used for bombardment with the Biolistic PSD-1000/He system operated at 900 psi, 1100 psi, 1300 psi or 1550 psi.
The target tissues used were callus and whole leaves from Anthurium andreanum and protocorms from Oncidium and Brassolaeliocattleya which are highly regenerative.
Transient GUS expression was monitored 24 h or 48 h post-bombardment.
The highest transient expression of GUS in A. andreanum callus tissues were obtained using pUGCI. This indicates that the promoter from maize, a monocot, is more efficient in expressing the GUS gene in Anthurium than dicot promoters.
In Oncidium, both pUGCI and pEF-1 gave high GUS activity.
For Brassolaeliocattleya protocorms, pEF-1 and pUGCI gave similar GUS activity per protocorm.
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