ISHS Acta Horticulturae 461: International Symposium on Biotechnology of Tropical and Subtropical Species Part 2
BREEDING OF RESISTANT PEPPER LINES (CAPSICUM ANNUUM L.) TO BACTERIAL SPOT (XANTHOMONAS CAMPESTRIS PV. VESICATORIA) THROUGH ANTHER CULTURE |
| Authors: | J.K. Hwang, K.Y. Paek, D.H. Cho |
| Keywords: | Capsicum annuum, bacterial spot resistance, anther culture |
| Abstract:
Experiments were conducted to obtain pepper lines resistant to bacterial spot with superior horticultural characteristics using anther culture techniques. Thirty three breeding lines and plants were derived from anthers of 3 F1 combinations. Nine lines including PI 163184 were shown to be resistant to the disease and PI 183439 and PI 244669 were moderately resistant, but others were susceptible. Pretreatment of anthers at 35 °C for 8 days under darkness was more effective than 4°C treatment for plant regeneration. Anthers at late uninucleate to binucleate stage responded better than those at other stages in inbred line-1-1xPI 163292 and inbred line -5 xPI 183481 combinations, but in MS line-3 x PI 183431, anthers at early uninucleate stage were more responsive. Large variations in the length of main stem and floral bud were observed with plants derived from anthers, and 2 chimeral plants with partial albino leaves were also observed. Most of the anther-derived plants were moderately resistant to the bacterial spot, and only a few plants were susceptible. | |
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