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ISHS Acta Horticulturae 461: International Symposium on Biotechnology of Tropical and Subtropical Species Part 2

MICROPROPAGATION AND IN VITRO CULTURE OF WILD RICE SPECIES

Authors:   A. Baset, S. Tobita, C. Li, S. Yashima, T. Senboku
Keywords:   callus culture, Oryza australiensis, Oryza eichingeri, Oryza granulata, Oryza latifolia, Oryza officinalis and Oryza rufipogon, plant regeneration, protoplast culture
Abstract:
A wide range of wild rice species (Oryza australiensis, O. eichingeri, O. granulata, O. latifolia, O. officinalis and O. rufipogon) were investigated with the ultimate aim of transferring their useful traits to cultivated rice varieties. A micropropagation system has been established for a number of wild rices, adopting an appropriate combination of plant hormones and culture conditions. According to the number of in vitro-propagated shoots in the system, O. eichingeri was most responsive and O. latifolia showed the least responsiveness. Embryogenic callus was successfully induced from the basal segments of micro-propagated shoots of some wild rice species, from which green and fertile plants were regenerated.

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