ISHS Acta Horticulturae 459: International Symposium Brassica 97, Xth Crucifer Genetics Workshop
EFFECTS OF TRANSGENIC OILSEED RAPE EXPRESSING PROTEINASE INHIBITORS ON PEST AND BENEFICIAL INSECTS |
| Authors: | M. Bonadé Bottino, C. Girard, L. Jouanin, M. Le Métayer, A.L. Picard-Nizou, G. Sandoz, M.H. Pham-Delègue, J. Lerin |
| Keywords: | Psylliodes chrysocephala, Ceutorynchus assimilis, Baris coerulescens, Apis mellifera |
| Abstract:
Crop protection against insect pests relies almost exclusively on the use of agrochemicals. Recent advances in plant genetic engineering offer opportunities for the creation of insect tolerant plants by insertion and expression in planta of entomopathogenic proteins. Oilseed rape is the target of numerous insects, mainly Coleopteran in France. In order to test the possibility of using proteinase inhibitors to confer insect pests resistance to this crop, transgenic oilseed rape lines constitutively expressing the serine proteinase inhibitor CII or the cysteine proteinase inhibitor OC-I have been obtained. The effects of ingestion of oilseed rape expressing OC-I have been determined on several Coleopteran pest larvae. Although no deleterious effect were observed on the insects tested, various reactions were observed at the gut proteinases level. Results are discussed together with prospects to improve pest resistance in rapeseed. In addition, since oilseed rape flowers provide abundant nectar and pollen and are therefore highly attractive to forager insects, possible deleterious effects on honeybees were assessed. Diet incorporation bioassays showed that deleterious effects were observed only at PI concentrations 100 fold higher than those expected in transgenic plants. | |
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