ISHS Acta Horticulturae 459: International Symposium Brassica 97, Xth Crucifer Genetics Workshop
REDUCTION OF CHLOROPHYLL ACCUMULATION IN SEED OF TRANSGENIC BRASSICA NAPUS USING ANTISENSE TECHNOLOGY |
| Authors: | J.C. Politeski Morissette, M.N. Konschuh, A.M. Johnson-Flanagan, J. Singh, L. Robert |
| Keywords: | cab expression, canola, green seed |
| Abstract:
Canola (Brassica napus cv. Westar) cotyledons have been transformed with an antisense construct of a type I cab gene of Photosystem II in an attempt to achieve the industry priority of reducing green seed. A napin promoter was used to target antisense gene expression to the seed. Northern blot analyses indicate that sense cab mRNA is suppressed during seed development in transgenic seed compared to seed from control plants. This results in significant reductions in cab protein and chlorophyll. The results of the study support the use of antisense cab as an effective means of reducing chlorophyll accumulation in seed of transgenic canola. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
